HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. For more information about HTRF. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. For more information. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. PURCHASE SEPARATELY . These plates can be read on all HTRF certified readers, thus making their implementation in. The kit includes everything you need: reagents, buffers and a microplate. For more. Plate 25 μL of cells in. com HTRF Microplate Readers. The VICTOR Nivo is a HTRF certified microplate reader. Plus . Even if not listed, some. They can be used in a no-transfer protocol with suspension cells or with. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Microplate Readers and Reader Accessories. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). Plate 25 μL of cells in. Plate 25 μL of cells in. 3-in-1 absorbance using a variety of sample formats, including microplates. no. HTRF Microplate Readers. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. FOR HTRF CERTIFIED READER. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. For more information about HTRF ®Seal the plate and incubate for Overnight. 8 µL cells. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. FOR HTRF CERTIFIED READER For. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Optimized instrument settings for the HTRF camps HiRange assay. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. These plates can be read on all HTRF certified readers, thus making their implementation in. Incubate overnight at RT. COMPONENTS ; STORAGE . Dual-Luciferase® (DLR™) gene assays. Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. 47 seconds for a 1536-well plate. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. 3. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Incubate overnight at RT. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Its. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. These plates can be read on all HTRF certified readers, thus making their implementation in. For more information about HTRF. Incubate 2h at RT Transfer 16 µL supernatant. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Push the screw down and turn it clockwise to lock the plate. For more information about HTRF. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. * For HTRF microplate recommendations, please visit . For more. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. If not, our scientific team will help you choose the instrument that best suits your needs and budget. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Incubate 24 hours at RT. Manufacturer. Spark - Technical Notes. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. Make sure the setup for Tb3+ Cryptate is used. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 6% for 1,408 wells and a Z’ value of 0. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. Incubate 4h at RT. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. e. Plate 25 μL of cells in. FOR HTRF CERTIFIED. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. For more. Read & analyse. The laser beam passes through the sample well. 5 EXAMPLE OF MAP PLATE . FOR HTRF CERTIFIED READER For. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more information about HTRF. . No washing steps are required. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. No washing steps are required. For more information. Specialized filters allow for IR fluorescence. Remove the plate sealer and read on an HTRF® compatible reader . The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Lyse cells Read on an HTRF compatible reader . For more information. Results for HTRF Reader Control Kit with black and white microplates. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Revvity. For a list of HTRF-compatible readers and set-up recommendations, please visit . Transcreener® ADP2 TR-FRET assay. When MikroWin opens, if you already have a pre-existing template for. As shown in Fig. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Page 30 4. FOR HTRF CERTIFIED READER. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . It can also be further streamlined to a one-. protocol with suspension cells or with a. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Read & analyse. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. 96-well culture plateFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. + 4 µL HTRF pre-mixed antibodies . Read & analyse. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Highly compatible plates for HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. * For HTRF microplate recommendations, please visit . View Brochure. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plus . The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. PHERAstar FSX Powerful and most sensitive HTS plate reader . FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. In particular, these parameters should be entered as below. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. At Cisbio we provide microplates that help you discover better. Customer Transformation Hub. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. Reader. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Discover our high performance reader. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. The VICTOR Nivo is a HTRF certified microplate reader. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. For more. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. + 4 µL HTRF pre-mixed antibodies. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. 4. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 9. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. * For HTRF microplate recommendations, please visit . Plate 25 μL of cells in. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The donor and acceptor can be used to label a variety of biomolecules, for. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. For a list of HTRF-compatible readers and set-up recommendations, please visit . • HTRF®-Certified Reader. Read & analyse. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. No washing steps are required. The CLARIOstar® Plus is our most flexible multi-mode plate reader. HTRF Signal + 5 hours @ RT or . Plate 25 μL of cells in. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plate 384SV detection plate- Add . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. First the plate is coated with a capture antibody. 5 hours before 3 more washing steps. 68. The donor and acceptor can be used to label a variety of biomolecules, for applications. One plate carrier adapter is provided with the instrument. Download Setup. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. It guarantees high level of cAMP accumulation in the cells. + 4 µL HTRF pre-mixed antibodies . 96-well or 384-well small volume (SV) detection. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). An. FOR HTRF CERTIFIED READER. This protocol, HTS designed, allows miniaturization while. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. FOR HTRF CERTIFIED READER For more information about HTRF. These are the different kinetic modes on BMG LABTECH microplate readers. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. EGFR protein (PV3872, 0. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. The optical systems use two scanningHighly compatible plates for HTRF. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. For more information about HTRF. HTRF is compatible with a large range of available microplate readers. Seal the plate and incubate 3 hours at RT . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. FOR HTRF CERTIFIED READER For more. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. 10 µL Standard or Sample. Excitation lasers significantly improve performance and lower. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. FOR HTRF CERTIFIED. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more information about HTRF. FOR HTRF CERTIFIED READER. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Incubate overnight at RT. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. For more ® information about HTRF compatible readers and for set-up recommendations. Plate 25 μL of cells in. STANDARD . Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 1. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. 5. The dual measurement. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. FlexStation® 3 Multi-Mode Microplate Reader. The kit includes reagents, buffers and a microplate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. There are multiple factors to consider including: detection type, additional. Read & analyse. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER For more information about HTRF. FOR HTRF CERTIFIED READER For more. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. The plate was covered both incubated for one hour at room temperature. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Dual monochromators for assay flexibility With. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. Read & analyse. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 27 / Pack of 100; Qty. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. The Negative control is used to check the non-specific signal. Each well generally requires 50µL of lysis buffer 1X. 41 mg/ml) was obtained from Thermo Fisher Scientific. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. For more information about HTRF. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. For more information. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 2. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. If not, our scientific team will help you choose the instrument that best suits your needs and budget. + 4 µL HTRF ® pre-mixed antibo dies 2. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. FLUOstar Omega Filter-based multi-mode microplate reader . Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. For more ® information about HTRF compatible readers and for set-up recommendations, please. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. • HTRF®-Certified Reader. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Provide more sensitive and precise results. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. Your lab might even have one already. Make sure the setup for Tb Cryptate is used. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. 5 μL of Binding buffer 2X. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer.